Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Representative images for immunohistochemical expression of Ki-67 ( A ), p63 ( B ), p53 ( C ) and Podoplanin ( D ) in leukoplakia. QuPath: Open source software for digital pathology image analysis (version 0.2.0-m4) was used to create the figure.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Immunohistochemical staining, Expressing, Software

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Representative images for the immunoexpression and boxplots for percentage of positive cells in recurring versus non-recurring leukoplakia for Ki-67 ( A ), p63 ( B ), p53 ( C ) and podoplanin (d240) ( D ). Box plot graphs showed median (horizontal bars), interquartile range, and maximum and minimum values (whiskers). QuPath: Open source software for digital pathology image analysis (version 0.2.0-m4) and Stata Statistical Software (release 16) were used to create the figure.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Software

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Kaplan–Meier analyses charting the cumulative probability of recurrence of leukoplakia divided by the median expression of Ki-67 ( A ), p63 ( B ), p53 ( C ) and podoplanin ( D ). Graphs show a trend that the high expression group of all four biomarkers by themselves are associated with recurrence after surgical excisions. SPSS Statistic (for Macintosh ver. 25.0 software package) was used to create the figure.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Expressing, Software

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Cox regression analyses based on expression of biomarkers as continuous variables and clinical and pathological factors for the recurrence of leukoplakia.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Expressing, Biomarker Discovery

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Kaplan–Meier analysis showing the cumulative probability of recurrence of leukoplakia. Leukoplakia were divided into high and low expression groups using the combined expression of p53 and p63 as described in the method section. The high expression group was found to have more recurrence hazards as compared to the low expression group (Log Rank, ( p = 0.036). Stata Statistical Software (release 16) was used to create the figure.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Expressing, Software

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Cox regression analyses based on the combined expression of p63 and p53.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia

doi: 10.1038/s41598-021-99326-5

Figure Lengend Snippet: Clone and dilutions of antibodies.

Article Snippet: Polyclonal rabbit antibody anti-human p63 primary antibody—HPA 006288, ATLAS ANTIBODIES, Sweden , 1:100.

Techniques: Clone Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63‐mediated transcription of KRT10 to improve psoriasis

doi: 10.1111/jcmm.15160

Figure Lengend Snippet: Tp63 is involved in ozone‐mediated KC cell differentiation in vitro. A and B, the protein levels of Tp63 were examined in blank, IMQ, IMQ + Vehicle and IMQ + Ozone groups by IHC staining (A) and immunoblotting (B). C and D, The protein levels of Tp63 were examined in normal skin samples and psoriasis lesions with or without the ozone therapy by IHC staining (C) and immunoblotting (D). E and F, KCs were stimulated with IL‐22 and examined for the mRNA expression (F) and protein levels of Tp63 (E) with or without ozone treatment. G, ChIP assays were performed in 293T cells with anti‐Tp63 antibody to validate the binding of Tp63 to KRT10 promoter region. H, Luciferase reporter assays were performed in 293T cells to validate the effects of Tp63 on KRT10 transcription activity. I and J, KCs were transfected with si‐Tp63 in the presence or absence of ozone treatment and examined for the mRNA expression and protein levels of Tp63 and KRT10 by real‐time PCR (I) and immunoblotting (J). * P < .05, ** P < .01, compared to the control group; # P < .05, ## P < .01, compared to the non‐treated group

Article Snippet: To evaluate the protein contents of IL‐17, IL‐22, Tp63, KRT6 and KRT10, tissue sections were fixed in acetone for 10 minutes at −20°C, permeabilized with 0.2% triton (Sigma) for 10 minutes at room temperature, incubated with a blocking solution (3.75% BSA/5% goat serum, Zymed) for 30 minutes and incubated for 2 hours with IL‐17 (26163‐1‐AP; Proteintech), IL‐22(ab203211; Abcam), Tp63 (PA2056; Bosterbio), KRT6 (10590‐1‐AP; Proteintech) or KRT10 (sc‐53252; Santa Cruz).

Techniques: Cell Differentiation, In Vitro, Immunohistochemistry, Western Blot, Expressing, Binding Assay, Luciferase, Activity Assay, Transfection, Real-time Polymerase Chain Reaction, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63‐mediated transcription of KRT10 to improve psoriasis

doi: 10.1111/jcmm.15160

Figure Lengend Snippet: Expression and correlation of Tp63 and KRT10 in tissue samples in vivo. A and B, The expression of Tp63 and KRT10 in psoriasis lesion tissues with or without ozone treatment were determined by real‐time PCR. C, The correlation of Tp63 and KRT10 expression in tissue samples was analysed by Pearson's correlation analysis

Article Snippet: To evaluate the protein contents of IL‐17, IL‐22, Tp63, KRT6 and KRT10, tissue sections were fixed in acetone for 10 minutes at −20°C, permeabilized with 0.2% triton (Sigma) for 10 minutes at room temperature, incubated with a blocking solution (3.75% BSA/5% goat serum, Zymed) for 30 minutes and incubated for 2 hours with IL‐17 (26163‐1‐AP; Proteintech), IL‐22(ab203211; Abcam), Tp63 (PA2056; Bosterbio), KRT6 (10590‐1‐AP; Proteintech) or KRT10 (sc‐53252; Santa Cruz).

Techniques: Expressing, In Vivo, Real-time Polymerase Chain Reaction